# DecodeME_fuma_supercluster Gene-Tissue Expression and Enrichment Report


## Clustering with k = 3


### Cluster 1 (k3)

**Genes (15):** VRK2 (sil: 0.26), HFE (sil: 0.22), TRIM38 (sil: 0.20), ZNF664 (sil: 0.20), ANKRD45 (sil: 0.14), GPR52 (sil: 0.14), SERPINC1 (sil: 0.14), MMS22L (sil: 0.09), POU3F2 (sil: 0.09), BTN2A2 (sil: 0.08), SOX6 (sil: 0.07), OLFM4 (sil: -0.01), BTN3A3 (sil: -0.05), PTGIS (sil: -0.06), PLCL1 (sil: -0.08)

This cluster contains **15 genes**.
Average Silhouette Score: **0.096**.
Highest expression is observed in: **choroid_plexus, oligodendrocyte_precursor, oligodendrocyte, committed_oligodendrocyte_precursor, ependymal**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**STRING network enrichment:**
*(Thresholds: FDR < 0.05. Showing top 5 terms per category.)*
- SMART: Domain in SPla and the RYanodine Receptor. (SM00449) (FDR: 0.007); Associated with SPRY domains (SM00589) (FDR: 0.007) *(Total significant: 2)*
- PMID: (1997) A 1.1-Mb transcript map of the hereditary hemochromatosis locus. (PMID:9149941) (FDR: 0.004) *(Total significant: 1)*
- HPO: Wellbeing measurement (EFO:0007869) (FDR: 1.14e-06); Intelligence (EFO:0004337) (FDR: 0.003); Mental or behavioural disorder biomarker (EFO:0006848) (FDR: 0.003); Insomnia measurement (EFO:0007876) (FDR: 0.030) *(Total significant: 4)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Positive Regulation of Peroxisome Proliferator Activated Receptor Signaling Pathway (GO:0035360) (Adj. P: 0.047, Combined Score: 1993.61); Negative Regulation of Cardiac Muscle Cell Differentiation (GO:2000726) (Adj. P: 0.047, Combined Score: 1993.61); Regulation of Interleukin-1-Mediated Signaling Pathway (GO:2000659) (Adj. P: 0.047, Combined Score: 1542.87); Cellular Response to Iron Ion (GO:0071281) (Adj. P: 0.047, Combined Score: 1542.87); Negative Regulation of Cardiocyte Differentiation (GO:1905208) (Adj. P: 0.047, Combined Score: 1542.87) ... *(Total significant: 68. See enrichr_terms.csv for full list.)*
- Reactome_2022: Butyrophilin (BTN) Family Interactions R-HSA-8851680 (Adj. P: 0.002, Combined Score: 3157.45) *(Total significant: 1)*
- Human_Phenotype_Ontology: Testicular atrophy (HP:0000029) (Adj. P: 0.048, Combined Score: 1043.43); Reduced antithrombin III activity (HP:0001976) (Adj. P: 0.048, Combined Score: 892.01); Deep venous thrombosis (HP:0002625) (Adj. P: 0.048, Combined Score: 892.01); Arthropathy (HP:0003040) (Adj. P: 0.048, Combined Score: 776.21); Azoospermia (HP:0000027) (Adj. P: 0.048, Combined Score: 551.00) ... *(Total significant: 11. See enrichr_terms.csv for full list.)*
- OMIM_Disease: alzheimer disease (Adj. P: 0.032, Combined Score: 225.04); hypertension (Adj. P: 0.032, Combined Score: 195.39) *(Total significant: 2)*


### Cluster 2 (k3)

**Genes (8):** SLC9C2 (sil: 0.18), DNAH10 (sil: 0.05), ZNF311 (sil: 0.05), PRDX6 (sil: 0.05), H4C8 (sil: 0.05), LRRIQ3 (sil: 0.02), SLC2A14 (sil: -0.10), SHISA6 (sil: -0.11)

This cluster contains **8 genes**.
Average Silhouette Score: **0.023**.
Highest expression is observed in: **ependymal, hippocampal_ca4, hippocampal_dentate_gyrus, thalamic_excitatory, lower_rhombic_lip**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Excitatory Chemical Synaptic Transmission (GO:0098976) (Adj. P: 0.029, Combined Score: 3445.29); Regulation of Short-Term Neuronal Synaptic Plasticity (GO:0048172) (Adj. P: 0.029, Combined Score: 1385.98); Dehydroascorbic Acid Transport (GO:0070837) (Adj. P: 0.029, Combined Score: 1385.98); D-glucose Import (GO:0046323) (Adj. P: 0.029, Combined Score: 961.58); Cilium Movement Involved in Cell Motility (GO:0060294) (Adj. P: 0.029, Combined Score: 725.58) ... *(Total significant: 11. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Dehydroascorbic Acid Transmembrane Transporter Activity (GO:0033300) (Adj. P: 0.013, Combined Score: 2797.66); Potassium:Proton Antiporter Activity (GO:0015386) (Adj. P: 0.013, Combined Score: 1549.44); Sodium:Proton Antiporter Activity (GO:0015385) (Adj. P: 0.013, Combined Score: 1251.42); Ionotropic Glutamate Receptor Binding (GO:0035255) (Adj. P: 0.013, Combined Score: 1138.86); Solute:Potassium Antiporter Activity (GO:0022821) (Adj. P: 0.013, Combined Score: 1043.43) ... *(Total significant: 10. See enrichr_terms.csv for full list.)*
- GO_Cellular_Component_2025: Asymmetric, Glutamatergic, Excitatory Synapse (GO:0098985) (Adj. P: 0.021, Combined Score: 4436.86); Dendritic Spine Membrane (GO:0032591) (Adj. P: 0.021, Combined Score: 2797.66); AMPA Glutamate Receptor Complex (GO:0032281) (Adj. P: 0.029, Combined Score: 643.51); Excitatory Synapse (GO:0060076) (Adj. P: 0.029, Combined Score: 576.75); Dendrite Membrane (GO:0032590) (Adj. P: 0.029, Combined Score: 454.45) ... *(Total significant: 8. See enrichr_terms.csv for full list.)*
- MSigDB_Hallmark_2020: Reactive Oxygen Species Pathway (Adj. P: 0.039, Combined Score: 233.90) *(Total significant: 1)*


### Cluster 3 (k3)

**Genes (36):** CCDC92 (sil: 0.39), HMGN4 (sil: 0.39), HTT (sil: 0.38), FBXL4 (sil: 0.38), CCPG1 (sil: 0.37), CDK5RAP1 (sil: 0.37), STAU1 (sil: 0.36), CSE1L (sil: 0.36), DDX27 (sil: 0.35), DARS2 (sil: 0.34), MRPL39 (sil: 0.32), SUDS3 (sil: 0.31), CACNA1E (sil: 0.31), ARFGEF2 (sil: 0.30), RC3H1 (sil: 0.30), LRRC7 (sil: 0.30), KLHL20 (sil: 0.28), RABGAP1L (sil: 0.28), ABT1 (sil: 0.28), MLLT10 (sil: 0.28) … *(Showing top 20. See cluster_k3_cluster_3_genes.txt for full list.)*

This cluster contains **36 genes**.
Average Silhouette Score: **0.277**.
Highest expression is observed in: **amygdala_excitatory, deep_layer_intratelencephalic, deep_layer_near_projecting, hippocampal_ca4, deep_layer_corticothalamic_and_6b**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**STRING network enrichment:**
*(Thresholds: FDR < 0.05. Showing top 5 terms per category.)*
- PMID: (2010) Identification of two novel chromosome regions associated with isolated growth hormone deficiency. (PMID:21284329) (FDR: 0.016) *(Total significant: 1)*
- HPO: Longevity (EFO:0004300) (FDR: 2.70e-04); Anthropometric measurement (EFO:0004302) (FDR: 2.70e-04); Intelligence (EFO:0004337) (FDR: 2.70e-04); Wellbeing measurement (EFO:0007869) (FDR: 2.70e-04); Cognitive function measurement (EFO:0008354) (FDR: 2.70e-04) ... *(Total significant: 17. See string_terms.csv for full list.)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Negative Regulation of T-helper Cell Differentiation (GO:0045623) (Adj. P: 0.035, Combined Score: 1524.87) *(Total significant: 1)*
- GO_Cellular_Component_2025: Cytoplasmic Stress Granule (GO:0010494) (Adj. P: 0.020, Combined Score: 171.40) *(Total significant: 1)*
- MSigDB_Hallmark_2020: Spermatogenesis (Adj. P: 0.024, Combined Score: 86.14) *(Total significant: 1)*
- OMIM_Disease: microcephaly (Adj. P: 0.043, Combined Score: 161.90); myocardial infarction (Adj. P: 0.043, Combined Score: 135.29) *(Total significant: 2)*


## Clustering with k = 11


### Cluster 1 (k11)

**Genes (4):** HFE (sil: 0.21), VRK2 (sil: 0.18), ZNF664 (sil: 0.12), TRIM38 (sil: 0.11)

This cluster contains **4 genes**.
Average Silhouette Score: **0.157**.
Highest expression is observed in: **oligodendrocyte, microglia, vascular, committed_oligodendrocyte_precursor, fibroblast**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Regulation of Interleukin-1-Mediated Signaling Pathway (GO:2000659) (Adj. P: 0.012, Combined Score: 8963.74); Cellular Response to Iron Ion (GO:0071281) (Adj. P: 0.012, Combined Score: 8963.74); Negative Regulation of CD8-positive, Alpha-Beta T Cell Activation (GO:2001186) (Adj. P: 0.012, Combined Score: 7298.30); Negative Regulation of T Cell Cytokine Production (GO:0002725) (Adj. P: 0.012, Combined Score: 6128.34); Positive Regulation of Peptide Secretion (GO:0002793) (Adj. P: 0.012, Combined Score: 6128.34) ... *(Total significant: 73. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Transferrin Receptor Binding (GO:1990459) (Adj. P: 0.020, Combined Score: 4600.93) *(Total significant: 1)*
- GO_Cellular_Component_2025: MHC Class I Protein Complex (GO:0042612) (Adj. P: 0.018, Combined Score: 11508.86); HFE-transferrin Receptor Complex (GO:1990712) (Adj. P: 0.018, Combined Score: 7298.30); MHC Protein Complex (GO:0042611) (Adj. P: 0.030, Combined Score: 1935.63); Lumenal Side of Endoplasmic Reticulum Membrane (GO:0098553) (Adj. P: 0.030, Combined Score: 1084.94); Phagocytic Vesicle Membrane (GO:0030670) (Adj. P: 0.030, Combined Score: 657.83) ... *(Total significant: 17. See enrichr_terms.csv for full list.)*
- Human_Phenotype_Ontology: Testicular atrophy (HP:0000029) (Adj. P: 0.014, Combined Score: 6128.34); Arthropathy (HP:0003040) (Adj. P: 0.014, Combined Score: 4600.93); Azoospermia (HP:0000027) (Adj. P: 0.014, Combined Score: 3304.67); Abnormal glucose tolerance (HP:0001952) (Adj. P: 0.014, Combined Score: 3012.37); Cerebral palsy (HP:0100021) (Adj. P: 0.014, Combined Score: 3012.37) ... *(Total significant: 26. See enrichr_terms.csv for full list.)*
- OMIM_Disease: alzheimer disease (Adj. P: 0.010, Combined Score: 1400.90); diabetes (Adj. P: 0.015, Combined Score: 377.38) *(Total significant: 2)*


### Cluster 2 (k11)

**Genes (2):** SOX6 (sil: 0.20), POU3F2 (sil: 0.17)

This cluster contains **2 genes**.
Average Silhouette Score: **0.185**.
Highest expression is observed in: **oligodendrocyte_precursor, ependymal, bergmann_glia, astrocyte, mge_interneuron**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*


### Cluster 3 (k11)

**Genes (3):** BTN2A2 (sil: 0.16), BTN3A3 (sil: -0.00), MMS22L (sil: -0.01)

This cluster contains **3 genes**.
Average Silhouette Score: **0.050**.
Highest expression is observed in: **midbrain_derived_inhibitory, lower_rhombic_lip, ependymal, microglia, splatter**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**STRING network enrichment:**
*(Thresholds: FDR < 0.05. Showing top 5 terms per category.)*
- SMART: Domain in SPla and the RYanodine Receptor. (SM00449) (FDR: 0.017); Associated with SPRY domains (SM00589) (FDR: 0.017) *(Total significant: 2)*
- RCTM: Butyrophilin (BTN) family interactions (HSA-8851680) (FDR: 0.003) *(Total significant: 1)*
- NetworkNeighborAL: Butyrophilin (BTN) family interactions, and Blepharitis (CL:31959) (FDR: 0.005) *(Total significant: 1)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: T Cell Receptor Signaling Pathway (GO:0050852) (Adj. P: 9.63e-04, Combined Score: 4523.03); Lymphocyte Mediated Immunity (GO:0002449) (Adj. P: 0.011, Combined Score: 3729.25); Antigen Receptor-Mediated Signaling Pathway (GO:0050851) (Adj. P: 9.63e-04, Combined Score: 3284.05); T Cell Mediated Immunity (GO:0002456) (Adj. P: 0.011, Combined Score: 3054.59); Adaptive Imm Resp Based on Som Recomb of Imm Rcptrs Built Frm IgSF Domains (GO:0002460) (Adj. P: 0.011, Combined Score: 2443.77) ... *(Total significant: 19. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Single-Stranded DNA Binding (GO:0003697) (Adj. P: 0.014, Combined Score: 438.96) *(Total significant: 1)*
- Reactome_2022: Butyrophilin (BTN) Family Interactions R-HSA-8851680 (Adj. P: 2.97e-06, Combined Score: 55267.79); Adaptive Immune System R-HSA-1280218 (Adj. P: 0.006, Combined Score: 292.02); Immune System R-HSA-168256 (Adj. P: 0.026, Combined Score: 67.57) *(Total significant: 3)*
- MSigDB_Hallmark_2020: E2F Targets (Adj. P: 0.030, Combined Score: 174.93) *(Total significant: 1)*


### Cluster 4 (k11)

**Genes (6):** ANKRD45 (sil: 0.40), GPR52 (sil: 0.40), SERPINC1 (sil: 0.40), OLFM4 (sil: 0.13), PTGIS (sil: 0.01), PLCL1 (sil: -0.03)

This cluster contains **6 genes**.
Average Silhouette Score: **0.219**.
Highest expression is observed in: **hippocampal_dentate_gyrus, midbrain_derived_inhibitory, choroid_plexus, oligodendrocyte, lower_rhombic_lip**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Positive Regulation of Peroxisome Proliferator Activated Receptor Signaling Pathway (GO:0035360) (Adj. P: 0.021, Combined Score: 6499.56); Negative Regulation of Nitric Oxide Biosynthetic Process (GO:0045019) (Adj. P: 0.021, Combined Score: 3445.29); Negative Regulation of Nitric Oxide Metabolic Process (GO:1904406) (Adj. P: 0.021, Combined Score: 3445.29); NAD Biosynthesis via Nicotinamide Riboside Salvage Pathway (GO:0034356) (Adj. P: 0.021, Combined Score: 2955.69); NAD Biosynthetic Process via the Salvage Pathway (GO:0034355) (Adj. P: 0.021, Combined Score: 2580.41) ... *(Total significant: 49. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: G Protein-Coupled Photoreceptor Activity (GO:0008020) (Adj. P: 0.026, Combined Score: 2044.88); Phosphatidylinositol Phospholipase C Activity (GO:0004435) (Adj. P: 0.026, Combined Score: 857.29); Phospholipase C Activity (GO:0004629) (Adj. P: 0.026, Combined Score: 775.88); Phosphoric Diester Hydrolase Activity (GO:0008081) (Adj. P: 0.033, Combined Score: 402.08); Serine-Type Endopeptidase Inhibitor Activity (GO:0004867) (Adj. P: 0.036, Combined Score: 271.98) ... *(Total significant: 8. See enrichr_terms.csv for full list.)*
- GO_Cellular_Component_2025: Primary Lysosome (GO:0005766) (Adj. P: 0.049, Combined Score: 2284.21) *(Total significant: 1)*
- Reactome_2022: Eicosanoids R-HSA-211979 (Adj. P: 0.028, Combined Score: 2044.88); Synthesis Of Bile Acids And Bile Salts Via 24-Hydroxycholesterol R-HSA-193775 (Adj. P: 0.028, Combined Score: 1682.80); Synthesis Of Prostaglandins (PG) And Thromboxanes (TX) R-HSA-2162123 (Adj. P: 0.028, Combined Score: 1542.87); Synthesis Of Bile Acids And Bile Salts Via 27-Hydroxycholesterol R-HSA-193807 (Adj. P: 0.028, Combined Score: 1542.87); Nicotinamide Salvaging R-HSA-197264 (Adj. P: 0.028, Combined Score: 1147.41) ... *(Total significant: 15. See enrichr_terms.csv for full list.)*
- Human_Phenotype_Ontology: Deep venous thrombosis (HP:0002625) (Adj. P: 0.008, Combined Score: 2955.69); Reduced antithrombin III activity (HP:0001976) (Adj. P: 0.008, Combined Score: 2955.69); Multifactorial inheritance (HP:0001426) (Adj. P: 0.017, Combined Score: 707.41); Pulmonary embolism (HP:0002204) (Adj. P: 0.020, Combined Score: 402.08) *(Total significant: 4)*
- OMIM_Disease: hypertension (Adj. P: 0.009, Combined Score: 677.12) *(Total significant: 1)*


### Cluster 5 (k11)

**Genes (3):** SLC9C2 (sil: 0.33), DNAH10 (sil: 0.32), LRRIQ3 (sil: 0.27)

This cluster contains **3 genes**.
Average Silhouette Score: **0.306**.
Highest expression is observed in: **ependymal, thalamic_excitatory, lower_rhombic_lip, deep_layer_corticothalamic_and_6b, medium_spiny_neuron**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Cilium Movement Involved in Cell Motility (GO:0060294) (Adj. P: 0.013, Combined Score: 3054.59); Sodium Ion Import Across Plasma Membrane (GO:0098719) (Adj. P: 0.013, Combined Score: 2574.28); Cilium-Dependent Cell Motility (GO:0060285) (Adj. P: 0.013, Combined Score: 2116.29); Cilium Movement (GO:0003341) (Adj. P: 0.024, Combined Score: 749.95); Sodium Ion Transmembrane Transport (GO:0035725) (Adj. P: 0.024, Combined Score: 502.79) ... *(Total significant: 10. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Potassium:Proton Antiporter Activity (GO:0015386) (Adj. P: 0.004, Combined Score: 6403.33); Sodium:Proton Antiporter Activity (GO:0015385) (Adj. P: 0.004, Combined Score: 5196.55); Solute:Potassium Antiporter Activity (GO:0022821) (Adj. P: 0.004, Combined Score: 4351.69); Metal Cation:Proton Antiporter Activity (GO:0051139) (Adj. P: 0.004, Combined Score: 2877.37); Sodium Ion Transmembrane Transporter Activity (GO:0015081) (Adj. P: 0.006, Combined Score: 1309.54) *(Total significant: 5)*
- GO_Cellular_Component_2025: Motile Cilium (GO:0031514) (Adj. P: 0.021, Combined Score: 517.55); 9+2 Motile Cilium (GO:0097729) (Adj. P: 0.021, Combined Score: 276.45) *(Total significant: 2)*
- Reactome_2022: Stimuli-sensing Channels R-HSA-2672351 (Adj. P: 0.039, Combined Score: 422.55); Ion Channel Transport R-HSA-983712 (Adj. P: 0.039, Combined Score: 207.85) *(Total significant: 2)*


### Cluster 6 (k11)

**Genes (3):** SHISA6 (sil: 0.07), PRDX6 (sil: 0.02), SLC2A14 (sil: 0.01)

This cluster contains **3 genes**.
Average Silhouette Score: **0.031**.
Highest expression is observed in: **hippocampal_ca4, hippocampal_dentate_gyrus, thalamic_excitatory, hippocampal_ca1_3, lower_rhombic_lip**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Excitatory Chemical Synaptic Transmission (GO:0098976) (Adj. P: 0.007, Combined Score: 14021.17); Regulation of Short-Term Neuronal Synaptic Plasticity (GO:0048172) (Adj. P: 0.007, Combined Score: 5741.92); Dehydroascorbic Acid Transport (GO:0070837) (Adj. P: 0.007, Combined Score: 5741.92); D-glucose Import (GO:0046323) (Adj. P: 0.007, Combined Score: 4018.42); Hexose Transmembrane Transport (GO:0008645) (Adj. P: 0.007, Combined Score: 2718.11) ... *(Total significant: 13. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Dehydroascorbic Acid Transmembrane Transporter Activity (GO:0033300) (Adj. P: 0.003, Combined Score: 11427.00); Ionotropic Glutamate Receptor Binding (GO:0035255) (Adj. P: 0.003, Combined Score: 4739.66); Hexose Transmembrane Transporter Activity (GO:0015149) (Adj. P: 0.003, Combined Score: 4018.42); Glutamate Receptor Binding (GO:0035254) (Adj. P: 0.003, Combined Score: 2877.37); D-glucose Transmembrane Transporter Activity (GO:0055056) (Adj. P: 0.003, Combined Score: 2718.11) *(Total significant: 5)*
- GO_Cellular_Component_2025: Asymmetric, Glutamatergic, Excitatory Synapse (GO:0098985) (Adj. P: 0.007, Combined Score: 17982.86); Dendritic Spine Membrane (GO:0032591) (Adj. P: 0.007, Combined Score: 11427.00); AMPA Glutamate Receptor Complex (GO:0032281) (Adj. P: 0.009, Combined Score: 2718.11); Excitatory Synapse (GO:0060076) (Adj. P: 0.009, Combined Score: 2443.77); Dendrite Membrane (GO:0032590) (Adj. P: 0.009, Combined Score: 1939.49) ... *(Total significant: 11. See enrichr_terms.csv for full list.)*
- Reactome_2022: Cellular Hexose Transport R-HSA-189200 (Adj. P: 0.009, Combined Score: 2877.37) *(Total significant: 1)*
- MSigDB_Hallmark_2020: Reactive Oxygen Species Pathway (Adj. P: 0.015, Combined Score: 1021.44); Fatty Acid Metabolism (Adj. P: 0.024, Combined Score: 236.95) *(Total significant: 2)*


### Cluster 7 (k11)

**Genes (2):** ZNF311 (sil: 0.14), H4C8 (sil: 0.11)

This cluster contains **2 genes**.
Average Silhouette Score: **0.122**.
Highest expression is observed in: **hippocampal_ca4, midbrain_derived_inhibitory, thalamic_excitatory, oligodendrocyte, deep_layer_intratelencephalic**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*


### Cluster 8 (k11)

**Genes (3):** CA10 (sil: 0.14), UNC13C (sil: 0.13), RABGAP1L (sil: -0.03)

This cluster contains **3 genes**.
Average Silhouette Score: **0.077**.
Highest expression is observed in: **upper_rhombic_lip, midbrain_derived_inhibitory, lower_rhombic_lip, hippocampal_dentate_gyrus, cerebellar_inhibitory**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Synaptic Vesicle Docking (GO:0016081) (Adj. P: 0.012, Combined Score: 8255.55); Synaptic Transmission, Glutamatergic (GO:0035249) (Adj. P: 0.018, Combined Score: 2216.11); Synaptic Vesicle Exocytosis (GO:0016079) (Adj. P: 0.019, Combined Score: 1198.68); Regulation of Cellular Localization (GO:0060341) (Adj. P: 0.023, Combined Score: 695.09); Regulation of Protein Localization (GO:0032880) (Adj. P: 0.026, Combined Score: 438.96) ... *(Total significant: 9. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Syntaxin-1 Binding (GO:0017075) (Adj. P: 0.016, Combined Score: 4351.69); Hydro-Lyase Activity (GO:0016836) (Adj. P: 0.016, Combined Score: 1103.68); Syntaxin Binding (GO:0019905) (Adj. P: 0.016, Combined Score: 1103.68); Small GTPase Binding (GO:0031267) (Adj. P: 0.039, Combined Score: 176.07); GTPase Activator Activity (GO:0005096) (Adj. P: 0.039, Combined Score: 160.20) ... *(Total significant: 6. See enrichr_terms.csv for full list.)*
- GO_Cellular_Component_2025: Neuromuscular Junction (GO:0031594) (Adj. P: 0.020, Combined Score: 1394.27); Exocytic Vesicle Membrane (GO:0099501) (Adj. P: 0.020, Combined Score: 695.09); Synaptic Vesicle Membrane (GO:0030672) (Adj. P: 0.020, Combined Score: 682.49) *(Total significant: 3)*
- MSigDB_Hallmark_2020: IL-2/STAT5 Signaling (Adj. P: 0.030, Combined Score: 176.07); KRAS Signaling Up (Adj. P: 0.030, Combined Score: 174.93) *(Total significant: 2)*


### Cluster 9 (k11)

**Genes (13):** RC3H1 (sil: 0.38), MLLT10 (sil: 0.37), HTT (sil: 0.33), ZNF644 (sil: 0.32), STAU1 (sil: 0.31), CCPG1 (sil: 0.29), ARFGEF2 (sil: 0.29), VSIG10 (sil: 0.27), FBXL4 (sil: 0.25), PEBP1 (sil: 0.19), CCDC92 (sil: 0.19), DNAJC1 (sil: 0.18), SUDS3 (sil: 0.07)

This cluster contains **13 genes**.
Average Silhouette Score: **0.266**.
Highest expression is observed in: **deep_layer_intratelencephalic, thalamic_excitatory, deep_layer_corticothalamic_and_6b, hippocampal_ca4, deep_layer_near_projecting**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Molecular_Function_2025: Double-Stranded RNA Binding (GO:0003725) (Adj. P: 0.025, Combined Score: 365.52) *(Total significant: 1)*
- GO_Cellular_Component_2025: Cytoplasmic Stress Granule (GO:0010494) (Adj. P: 0.022, Combined Score: 292.65) *(Total significant: 1)*
- MSigDB_Hallmark_2020: Spermatogenesis (Adj. P: 0.013, Combined Score: 154.59) *(Total significant: 1)*
- Human_Phenotype_Ontology: Abnormality of alanine metabolism (HP:0010916) (Adj. P: 0.047, Combined Score: 1251.42); Abnormality of pyruvate family amino acid metabolism (HP:0010915) (Adj. P: 0.047, Combined Score: 1251.42); Hyperalaninemia (HP:0003348) (Adj. P: 0.047, Combined Score: 1251.42); Tetraparesis (HP:0002273) (Adj. P: 0.047, Combined Score: 1070.48); Personality changes (HP:0000751) (Adj. P: 0.047, Combined Score: 469.12) ... *(Total significant: 26. See enrichr_terms.csv for full list.)*
- OMIM_Disease: microcephaly (Adj. P: 0.009, Combined Score: 602.21) *(Total significant: 1)*


### Cluster 10 (k11)

**Genes (6):** PCDH17 (sil: 0.24), DCC (sil: 0.23), LRRC7 (sil: 0.22), TAOK3 (sil: 0.12), ZBTB37 (sil: 0.05), CACNA1E (sil: 0.01)

This cluster contains **6 genes**.
Average Silhouette Score: **0.146**.
Highest expression is observed in: **medium_spiny_neuron, hippocampal_ca1_3, amygdala_excitatory, hippocampal_dentate_gyrus, hippocampal_ca4**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**STRING network enrichment:**
*(Thresholds: FDR < 0.05. Showing top 5 terms per category.)*
- HPO: Depressive symptom measurement (EFO:0007006) (FDR: 0.002); Wellbeing measurement (EFO:0007869) (FDR: 0.002); Neuroticism measurement (EFO:0007660) (FDR: 0.003); Cognitive function measurement (EFO:0008354) (FDR: 0.048) *(Total significant: 4)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Negative Regulation of Collateral Sprouting (GO:0048671) (Adj. P: 0.022, Combined Score: 6499.56); Neurotransmitter Receptor Transport, Endosome to Plasma Membrane (GO:0099639) (Adj. P: 0.022, Combined Score: 6499.56); Neurotransmitter Receptor Transport to Postsynaptic Membrane (GO:0098969) (Adj. P: 0.022, Combined Score: 5053.71); Regulation of Collateral Sprouting (GO:0048670) (Adj. P: 0.022, Combined Score: 4108.59); Positive Regulation of Stress-Activated MAPK Cascade (GO:0032874) (Adj. P: 0.032, Combined Score: 1422.78) ... *(Total significant: 32. See enrichr_terms.csv for full list.)*
- Reactome_2022: Netrin Mediated Repulsion Signals R-HSA-418886 (Adj. P: 0.021, Combined Score: 3445.29); Regulation Of Commissural Axon Pathfinding By SLIT And ROBO R-HSA-428542 (Adj. P: 0.021, Combined Score: 2955.69); Role Of Second Messengers In Netrin-1 Signaling R-HSA-418890 (Adj. P: 0.021, Combined Score: 2580.41); Caspase Activation Via Dependence Receptors In Absence Of Ligand R-HSA-418889 (Adj. P: 0.021, Combined Score: 2580.41); DSCAM Interactions R-HSA-376172 (Adj. P: 0.021, Combined Score: 2284.21) ... *(Total significant: 20. See enrichr_terms.csv for full list.)*
- Human_Phenotype_Ontology: Leiomyosarcoma (HP:0100243) (Adj. P: 0.013, Combined Score: 1076.21); Uterine leiomyosarcoma (HP:0002891) (Adj. P: 0.013, Combined Score: 1076.21); Transitional cell carcinoma of the bladder (HP:0006740) (Adj. P: 0.013, Combined Score: 955.53); Hereditary nonpolyposis colorectal carcinoma (HP:0006716) (Adj. P: 0.013, Combined Score: 904.00); Bladder carcinoma (HP:0002862) (Adj. P: 0.013, Combined Score: 857.29) ... *(Total significant: 11. See enrichr_terms.csv for full list.)*
- OMIM_Disease: colorectal cancer (Adj. P: 0.012, Combined Score: 453.10) *(Total significant: 1)*


### Cluster 11 (k11)

**Genes (14):** DDX27 (sil: 0.29), ZNF322 (sil: 0.29), CSE1L (sil: 0.25), ECI2 (sil: 0.23), KLHL20 (sil: 0.22), DARS2 (sil: 0.22), ZNFX1 (sil: 0.21), B4GALT5 (sil: 0.20), ABT1 (sil: 0.19), MRPL39 (sil: 0.17), TNFSF4 (sil: 0.12), CDK5RAP1 (sil: 0.11), HMGN4 (sil: 0.11), NEGR1 (sil: -0.04)

This cluster contains **14 genes**.
Average Silhouette Score: **0.184**.
Highest expression is observed in: **thalamic_excitatory, deep_layer_near_projecting, deep_layer_intratelencephalic, deep_layer_corticothalamic_and_6b, hippocampal_ca4**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- OMIM_Disease: myocardial infarction (Adj. P: 0.011, Combined Score: 460.53) *(Total significant: 1)*


## Clustering with k = 15


### Cluster 1 (k15)

**Genes (2):** ZNF664 (sil: 0.19), VRK2 (sil: 0.11)

This cluster contains **2 genes**.
Average Silhouette Score: **0.148**.
Highest expression is observed in: **oligodendrocyte, committed_oligodendrocyte_precursor, choroid_plexus, microglia, vascular**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*


### Cluster 2 (k15)

**Genes (2):** TRIM38 (sil: 0.17), HFE (sil: 0.06)

This cluster contains **2 genes**.
Average Silhouette Score: **0.115**.
Highest expression is observed in: **microglia, vascular, fibroblast, miscellaneous, oligodendrocyte**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*


### Cluster 3 (k15)

**Genes (2):** SOX6 (sil: 0.11), POU3F2 (sil: 0.07)

This cluster contains **2 genes**.
Average Silhouette Score: **0.090**.
Highest expression is observed in: **oligodendrocyte_precursor, ependymal, bergmann_glia, astrocyte, mge_interneuron**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*


### Cluster 4 (k15)

**Genes (2):** MMS22L (sil: 0.10), BTN2A2 (sil: 0.08)

This cluster contains **2 genes**.
Average Silhouette Score: **0.092**.
Highest expression is observed in: **midbrain_derived_inhibitory, ependymal, lower_rhombic_lip, oligodendrocyte, microglia**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*


### Cluster 5 (k15)

**Genes (1):** BTN3A3 (sil: 0.00)

This cluster contains **1 genes**.
Average Silhouette Score: **0.000**.
Highest expression is observed in: **midbrain_derived_inhibitory, cge_interneuron, miscellaneous, lower_rhombic_lip, deep_layer_near_projecting**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*


### Cluster 6 (k15)

**Genes (4):** ANKRD45 (sil: 0.67), GPR52 (sil: 0.67), SERPINC1 (sil: 0.67), OLFM4 (sil: 0.23)

This cluster contains **4 genes**.
Average Silhouette Score: **0.558**.
Highest expression is observed in: **upper_layer_intratelencephalic, amygdala_excitatory, lamp5_lhx6_and_chandelier, upper_rhombic_lip, thalamic_excitatory**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Positive Regulation of Substrate Adhesion-Dependent Cell Spreading (GO:1900026) (Adj. P: 0.015, Combined Score: 971.56); Cellular Response to Radiation (GO:0071478) (Adj. P: 0.015, Combined Score: 675.19); Regulation of Substrate Adhesion-Dependent Cell Spreading (GO:1900024) (Adj. P: 0.015, Combined Score: 595.71); Response to Light Stimulus (GO:0009416) (Adj. P: 0.015, Combined Score: 509.22); Cellular Response to Light Stimulus (GO:0071482) (Adj. P: 0.015, Combined Score: 478.73) ... *(Total significant: 6. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: G Protein-Coupled Photoreceptor Activity (GO:0008020) (Adj. P: 0.014, Combined Score: 3653.70); Serine-Type Endopeptidase Inhibitor Activity (GO:0004867) (Adj. P: 0.035, Combined Score: 498.68); Endopeptidase Inhibitor Activity (GO:0004866) (Adj. P: 0.035, Combined Score: 210.40); Protease Binding (GO:0002020) (Adj. P: 0.035, Combined Score: 210.40) *(Total significant: 4)*
- GO_Cellular_Component_2025: Primary Lysosome (GO:0005766) (Adj. P: 0.022, Combined Score: 4077.19); Tertiary Granule Lumen (GO:1904724) (Adj. P: 0.030, Combined Score: 568.46); Specific Granule Lumen (GO:0035580) (Adj. P: 0.030, Combined Score: 488.52); Azurophil Granule (GO:0042582) (Adj. P: 0.046, Combined Score: 149.69); Specific Granule (GO:0042581) (Adj. P: 0.046, Combined Score: 144.82) ... *(Total significant: 7. See enrichr_terms.csv for full list.)*
- Reactome_2022: Intrinsic Pathway Of Fibrin Clot Formation R-HSA-140837 (Adj. P: 0.024, Combined Score: 1822.54); Common Pathway Of Fibrin Clot Formation R-HSA-140875 (Adj. P: 0.024, Combined Score: 1720.94); Formation Of Fibrin Clot (Clotting Cascade) R-HSA-140877 (Adj. P: 0.027, Combined Score: 907.25) *(Total significant: 3)*
- MSigDB_Hallmark_2020: Coagulation (Adj. P: 0.039, Combined Score: 173.96); IL-2/STAT5 Signaling (Adj. P: 0.039, Combined Score: 107.95); Complement (Adj. P: 0.039, Combined Score: 107.24) *(Total significant: 3)*
- Human_Phenotype_Ontology: Deep venous thrombosis (HP:0002625) (Adj. P: 0.004, Combined Score: 5264.00); Reduced antithrombin III activity (HP:0001976) (Adj. P: 0.004, Combined Score: 5264.00); Pulmonary embolism (HP:0002204) (Adj. P: 0.015, Combined Score: 732.58) *(Total significant: 3)*


### Cluster 7 (k15)

**Genes (1):** PLCL1 (sil: 0.00)

This cluster contains **1 genes**.
Average Silhouette Score: **0.000**.
Highest expression is observed in: **oligodendrocyte, hippocampal_dentate_gyrus, midbrain_derived_inhibitory, choroid_plexus, lower_rhombic_lip**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*


### Cluster 8 (k15)

**Genes (1):** PTGIS (sil: 0.00)

This cluster contains **1 genes**.
Average Silhouette Score: **0.000**.
Highest expression is observed in: **fibroblast, deep_layer_near_projecting, splatter, medium_spiny_neuron, deep_layer_intratelencephalic**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*


### Cluster 9 (k15)

**Genes (3):** SLC9C2 (sil: 0.33), DNAH10 (sil: 0.32), LRRIQ3 (sil: 0.27)

This cluster contains **3 genes**.
Average Silhouette Score: **0.306**.
Highest expression is observed in: **ependymal, thalamic_excitatory, lower_rhombic_lip, deep_layer_corticothalamic_and_6b, medium_spiny_neuron**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Cilium Movement Involved in Cell Motility (GO:0060294) (Adj. P: 0.013, Combined Score: 3054.59); Sodium Ion Import Across Plasma Membrane (GO:0098719) (Adj. P: 0.013, Combined Score: 2574.28); Cilium-Dependent Cell Motility (GO:0060285) (Adj. P: 0.013, Combined Score: 2116.29); Cilium Movement (GO:0003341) (Adj. P: 0.024, Combined Score: 749.95); Sodium Ion Transmembrane Transport (GO:0035725) (Adj. P: 0.024, Combined Score: 502.79) ... *(Total significant: 10. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Potassium:Proton Antiporter Activity (GO:0015386) (Adj. P: 0.004, Combined Score: 6403.33); Sodium:Proton Antiporter Activity (GO:0015385) (Adj. P: 0.004, Combined Score: 5196.55); Solute:Potassium Antiporter Activity (GO:0022821) (Adj. P: 0.004, Combined Score: 4351.69); Metal Cation:Proton Antiporter Activity (GO:0051139) (Adj. P: 0.004, Combined Score: 2877.37); Sodium Ion Transmembrane Transporter Activity (GO:0015081) (Adj. P: 0.006, Combined Score: 1309.54) *(Total significant: 5)*
- GO_Cellular_Component_2025: Motile Cilium (GO:0031514) (Adj. P: 0.021, Combined Score: 517.55); 9+2 Motile Cilium (GO:0097729) (Adj. P: 0.021, Combined Score: 276.45) *(Total significant: 2)*
- Reactome_2022: Stimuli-sensing Channels R-HSA-2672351 (Adj. P: 0.039, Combined Score: 422.55); Ion Channel Transport R-HSA-983712 (Adj. P: 0.039, Combined Score: 207.85) *(Total significant: 2)*


### Cluster 10 (k15)

**Genes (3):** SHISA6 (sil: 0.07), PRDX6 (sil: 0.02), SLC2A14 (sil: 0.01)

This cluster contains **3 genes**.
Average Silhouette Score: **0.031**.
Highest expression is observed in: **hippocampal_ca4, hippocampal_dentate_gyrus, thalamic_excitatory, hippocampal_ca1_3, lower_rhombic_lip**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Excitatory Chemical Synaptic Transmission (GO:0098976) (Adj. P: 0.007, Combined Score: 14021.17); Regulation of Short-Term Neuronal Synaptic Plasticity (GO:0048172) (Adj. P: 0.007, Combined Score: 5741.92); Dehydroascorbic Acid Transport (GO:0070837) (Adj. P: 0.007, Combined Score: 5741.92); D-glucose Import (GO:0046323) (Adj. P: 0.007, Combined Score: 4018.42); Hexose Transmembrane Transport (GO:0008645) (Adj. P: 0.007, Combined Score: 2718.11) ... *(Total significant: 13. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Dehydroascorbic Acid Transmembrane Transporter Activity (GO:0033300) (Adj. P: 0.003, Combined Score: 11427.00); Ionotropic Glutamate Receptor Binding (GO:0035255) (Adj. P: 0.003, Combined Score: 4739.66); Hexose Transmembrane Transporter Activity (GO:0015149) (Adj. P: 0.003, Combined Score: 4018.42); Glutamate Receptor Binding (GO:0035254) (Adj. P: 0.003, Combined Score: 2877.37); D-glucose Transmembrane Transporter Activity (GO:0055056) (Adj. P: 0.003, Combined Score: 2718.11) *(Total significant: 5)*
- GO_Cellular_Component_2025: Asymmetric, Glutamatergic, Excitatory Synapse (GO:0098985) (Adj. P: 0.007, Combined Score: 17982.86); Dendritic Spine Membrane (GO:0032591) (Adj. P: 0.007, Combined Score: 11427.00); AMPA Glutamate Receptor Complex (GO:0032281) (Adj. P: 0.009, Combined Score: 2718.11); Excitatory Synapse (GO:0060076) (Adj. P: 0.009, Combined Score: 2443.77); Dendrite Membrane (GO:0032590) (Adj. P: 0.009, Combined Score: 1939.49) ... *(Total significant: 11. See enrichr_terms.csv for full list.)*
- Reactome_2022: Cellular Hexose Transport R-HSA-189200 (Adj. P: 0.009, Combined Score: 2877.37) *(Total significant: 1)*
- MSigDB_Hallmark_2020: Reactive Oxygen Species Pathway (Adj. P: 0.015, Combined Score: 1021.44); Fatty Acid Metabolism (Adj. P: 0.024, Combined Score: 236.95) *(Total significant: 2)*


### Cluster 11 (k15)

**Genes (2):** H4C8 (sil: 0.08), ZNF311 (sil: 0.07)

This cluster contains **2 genes**.
Average Silhouette Score: **0.073**.
Highest expression is observed in: **hippocampal_ca4, midbrain_derived_inhibitory, thalamic_excitatory, oligodendrocyte, deep_layer_intratelencephalic**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*


### Cluster 12 (k15)

**Genes (3):** UNC13C (sil: 0.13), CA10 (sil: 0.11), RABGAP1L (sil: -0.03)

This cluster contains **3 genes**.
Average Silhouette Score: **0.068**.
Highest expression is observed in: **upper_rhombic_lip, midbrain_derived_inhibitory, lower_rhombic_lip, hippocampal_dentate_gyrus, cerebellar_inhibitory**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Synaptic Vesicle Docking (GO:0016081) (Adj. P: 0.012, Combined Score: 8255.55); Synaptic Transmission, Glutamatergic (GO:0035249) (Adj. P: 0.018, Combined Score: 2216.11); Synaptic Vesicle Exocytosis (GO:0016079) (Adj. P: 0.019, Combined Score: 1198.68); Regulation of Cellular Localization (GO:0060341) (Adj. P: 0.023, Combined Score: 695.09); Regulation of Protein Localization (GO:0032880) (Adj. P: 0.026, Combined Score: 438.96) ... *(Total significant: 9. See enrichr_terms.csv for full list.)*
- GO_Molecular_Function_2025: Syntaxin-1 Binding (GO:0017075) (Adj. P: 0.016, Combined Score: 4351.69); Hydro-Lyase Activity (GO:0016836) (Adj. P: 0.016, Combined Score: 1103.68); Syntaxin Binding (GO:0019905) (Adj. P: 0.016, Combined Score: 1103.68); Small GTPase Binding (GO:0031267) (Adj. P: 0.039, Combined Score: 176.07); GTPase Activator Activity (GO:0005096) (Adj. P: 0.039, Combined Score: 160.20) ... *(Total significant: 6. See enrichr_terms.csv for full list.)*
- GO_Cellular_Component_2025: Neuromuscular Junction (GO:0031594) (Adj. P: 0.020, Combined Score: 1394.27); Exocytic Vesicle Membrane (GO:0099501) (Adj. P: 0.020, Combined Score: 695.09); Synaptic Vesicle Membrane (GO:0030672) (Adj. P: 0.020, Combined Score: 682.49) *(Total significant: 3)*
- MSigDB_Hallmark_2020: IL-2/STAT5 Signaling (Adj. P: 0.030, Combined Score: 176.07); KRAS Signaling Up (Adj. P: 0.030, Combined Score: 174.93) *(Total significant: 2)*


### Cluster 13 (k15)

**Genes (13):** RC3H1 (sil: 0.38), MLLT10 (sil: 0.37), HTT (sil: 0.33), STAU1 (sil: 0.31), CCPG1 (sil: 0.29), ARFGEF2 (sil: 0.29), VSIG10 (sil: 0.27), ZNF644 (sil: 0.26), FBXL4 (sil: 0.25), PEBP1 (sil: 0.19), CCDC92 (sil: 0.19), DNAJC1 (sil: 0.18), SUDS3 (sil: 0.07)

This cluster contains **13 genes**.
Average Silhouette Score: **0.261**.
Highest expression is observed in: **deep_layer_intratelencephalic, thalamic_excitatory, deep_layer_corticothalamic_and_6b, hippocampal_ca4, deep_layer_near_projecting**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Molecular_Function_2025: Double-Stranded RNA Binding (GO:0003725) (Adj. P: 0.025, Combined Score: 365.52) *(Total significant: 1)*
- GO_Cellular_Component_2025: Cytoplasmic Stress Granule (GO:0010494) (Adj. P: 0.022, Combined Score: 292.65) *(Total significant: 1)*
- MSigDB_Hallmark_2020: Spermatogenesis (Adj. P: 0.013, Combined Score: 154.59) *(Total significant: 1)*
- Human_Phenotype_Ontology: Abnormality of alanine metabolism (HP:0010916) (Adj. P: 0.047, Combined Score: 1251.42); Abnormality of pyruvate family amino acid metabolism (HP:0010915) (Adj. P: 0.047, Combined Score: 1251.42); Hyperalaninemia (HP:0003348) (Adj. P: 0.047, Combined Score: 1251.42); Tetraparesis (HP:0002273) (Adj. P: 0.047, Combined Score: 1070.48); Personality changes (HP:0000751) (Adj. P: 0.047, Combined Score: 469.12) ... *(Total significant: 26. See enrichr_terms.csv for full list.)*
- OMIM_Disease: microcephaly (Adj. P: 0.009, Combined Score: 602.21) *(Total significant: 1)*


### Cluster 14 (k15)

**Genes (6):** PCDH17 (sil: 0.24), DCC (sil: 0.23), LRRC7 (sil: 0.22), TAOK3 (sil: 0.12), ZBTB37 (sil: 0.05), CACNA1E (sil: 0.01)

This cluster contains **6 genes**.
Average Silhouette Score: **0.146**.
Highest expression is observed in: **medium_spiny_neuron, hippocampal_ca1_3, amygdala_excitatory, hippocampal_dentate_gyrus, hippocampal_ca4**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**STRING network enrichment:**
*(Thresholds: FDR < 0.05. Showing top 5 terms per category.)*
- HPO: Depressive symptom measurement (EFO:0007006) (FDR: 0.002); Wellbeing measurement (EFO:0007869) (FDR: 0.002); Neuroticism measurement (EFO:0007660) (FDR: 0.003); Cognitive function measurement (EFO:0008354) (FDR: 0.048) *(Total significant: 4)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- GO_Biological_Process_2025: Negative Regulation of Collateral Sprouting (GO:0048671) (Adj. P: 0.022, Combined Score: 6499.56); Neurotransmitter Receptor Transport, Endosome to Plasma Membrane (GO:0099639) (Adj. P: 0.022, Combined Score: 6499.56); Neurotransmitter Receptor Transport to Postsynaptic Membrane (GO:0098969) (Adj. P: 0.022, Combined Score: 5053.71); Regulation of Collateral Sprouting (GO:0048670) (Adj. P: 0.022, Combined Score: 4108.59); Positive Regulation of Stress-Activated MAPK Cascade (GO:0032874) (Adj. P: 0.032, Combined Score: 1422.78) ... *(Total significant: 32. See enrichr_terms.csv for full list.)*
- Reactome_2022: Netrin Mediated Repulsion Signals R-HSA-418886 (Adj. P: 0.021, Combined Score: 3445.29); Regulation Of Commissural Axon Pathfinding By SLIT And ROBO R-HSA-428542 (Adj. P: 0.021, Combined Score: 2955.69); Role Of Second Messengers In Netrin-1 Signaling R-HSA-418890 (Adj. P: 0.021, Combined Score: 2580.41); Caspase Activation Via Dependence Receptors In Absence Of Ligand R-HSA-418889 (Adj. P: 0.021, Combined Score: 2580.41); DSCAM Interactions R-HSA-376172 (Adj. P: 0.021, Combined Score: 2284.21) ... *(Total significant: 20. See enrichr_terms.csv for full list.)*
- Human_Phenotype_Ontology: Leiomyosarcoma (HP:0100243) (Adj. P: 0.013, Combined Score: 1076.21); Uterine leiomyosarcoma (HP:0002891) (Adj. P: 0.013, Combined Score: 1076.21); Transitional cell carcinoma of the bladder (HP:0006740) (Adj. P: 0.013, Combined Score: 955.53); Hereditary nonpolyposis colorectal carcinoma (HP:0006716) (Adj. P: 0.013, Combined Score: 904.00); Bladder carcinoma (HP:0002862) (Adj. P: 0.013, Combined Score: 857.29) ... *(Total significant: 11. See enrichr_terms.csv for full list.)*
- OMIM_Disease: colorectal cancer (Adj. P: 0.012, Combined Score: 453.10) *(Total significant: 1)*


### Cluster 15 (k15)

**Genes (14):** DDX27 (sil: 0.29), ZNF322 (sil: 0.29), CSE1L (sil: 0.25), ECI2 (sil: 0.23), KLHL20 (sil: 0.22), DARS2 (sil: 0.22), ZNFX1 (sil: 0.21), B4GALT5 (sil: 0.20), ABT1 (sil: 0.19), MRPL39 (sil: 0.17), TNFSF4 (sil: 0.12), CDK5RAP1 (sil: 0.11), HMGN4 (sil: 0.11), NEGR1 (sil: -0.04)

This cluster contains **14 genes**.
Average Silhouette Score: **0.184**.
Highest expression is observed in: **thalamic_excitatory, deep_layer_near_projecting, deep_layer_intratelencephalic, deep_layer_corticothalamic_and_6b, hippocampal_ca4**.
*(Showing top 5 tissues. Refer to full expression matrix for all values.)*

**Enrichr annotation enrichment:**
*(Thresholds: Adj. P < 0.05, Combined Score > 10. Showing top 5 terms per library.)*
- OMIM_Disease: myocardial infarction (Adj. P: 0.011, Combined Score: 460.53) *(Total significant: 1)*